Connectivirty and Contraction In Cytoskeleltal Networks

The cytoskeleton's ability to contract and propagate forces is the fundamental mechanism behind cell morphology, division and migration. This can only occur if the network is sufficiently connected, yet a rigorous description of the connectivity requirements has never been provided. In this work we focused on the polarity-sorting contraction mechanism and showed that connectivity is not determined by the spatial distribution of filaments alone, but by the interconnectivity between the dual network of filaments and motors. We developed a method to quantify filament-motor connectivity as a function of motor length, filament length distributions, and the densities of each component. WUsing this framework, we derived a general theory that predicts when a network is sufficiently connected to allow global or local contraction. We validated our predictions with computer simulations and introduced a novel metric to distinguish between these outcomes. Our findings show that the conditions for local and global contraction in the presence of fiber dynamics correspond, respectively, to the pulsatile and steady-state contraction behaviors observed in vivo. All results are independent of filament rigidity, making our conclusions applicable to both actin and microtubule networks. Lastly, we discuss how those outcomes are affected by the introduction of crosslinking proteins, which - despite not actively generating forces of their own - can promote global contractility at small concentrations even in networks made of short and/or rigid filaments.

Connectivirty and Contraction In Cytoskeleltal Networks

Monday, July 14 at 6:00pm in

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Julio Belmonte

"Connectivirty and Contraction In Cytoskeleltal Networks"

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